Bradley S. Carter, Christian Cortés-Campos, Xiao Chen, Jasmine M. McCammon, and Hazel L. Sive.
Zebrafish. February 2017, 14(1): 73-76. doi:10.1089/zeb.2016.1326. (full-text access for subscribers)
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.