Thursday, March 02, 2017

Brad Carter publishes in the journal Zebrafish

New publication from Brad Carter, Visiting Assistant Professor in Neuroscience, from research completed at the Whitehead Institute for Biomedical Research, Cambridge, Massachusetts.

Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.
Bradley S. Carter, Christian Cortés-Campos, Xiao Chen, Jasmine M. McCammon, and Hazel L. Sive.
Zebrafish. February 2017, 14(1): 73-76. doi:10.1089/zeb.2016.1326.  (full-text access for subscribers)

Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.

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